Figure 3

DENV infection increases microglial migration. (A) Time-lapse microscopy showed the kinetic tracking of cell migration in DENV 2-innoculated BV2 cells (n = 6) for 12 h. (B) Cell tracking analysis showed the velocity and distance of the migrated cells. (C) The wound-healing assay and (D) the measurement of the migrating cell number and wound area displayed cell migration. (E) The MTT and LDH assays showed cell viability and cytotoxicity, respectively, of cells inoculated with DENV. All quantitative data are shown as the means ± SD from three independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001. ns, not significant.