Figure 5

DENV infection increases cell migration through the TLR3/Src-regulated pathway. BV2 cells were inoculated with DENV 2 (MOI = 50) for 12 h in the absence or presence of UV irradiation (UVi). (A) The plaque assay confirmed virus release. (B) Wound-healing assay-based analysis of cell migration performed using the Image J software based on the number of migrating cells and wound area changes. (C) Representative fluorescent immunostaining images and MFI analysis displayed dsRNA replication. Migration in cells incubated with or without (D) TLR3 inhibitor (TLR3i, 5 µM) and (E) Src inhibitor (PP2, 25 µM), PI3K inhibitor (LY294002, 20 µM), or NF-kB inhibitor (CAPE, 0.1 μM) treatment was measured by the wound-healing assay 12 h post-infection. Poly(I:C) was used as a positive control for TLR3 activation. The quantitative data are depicted as the means ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001. ns, not significant.