Figure 3
From: Lysophosphatidic acid counteracts glucagon-induced hepatocyte glucose production via STAT3
PLPP1 KO mice have reduced pyruvate-stimulated hepatic gluconeogenesis without changes in insulin or glucose tolerance. (A) Body weights of mice on chow diet and throughout the course of HFD feeding. Mice were fed a normal chow diet until approximately 12 weeks of age and were switched to a HFD for 18 weeks. Body weights were monitored weekly and averaged, yielding weight values for each mouse for every 2 weeks; n = 16–21 per genotype. (B) Weights of tissues harvested from HFD-fed mice, expressed as percentage of total body weight for each mouse. RP (retroperitoneal) and SC (subcutaneous) fat; n = 4–20 per genotype. (C,D) Pyruvate tolerance tests (PTTs) in overnight-fasted mice fed a chow or HFD diet for 15–17 weeks; for chow n = 6–10 per genotype and for HFD n = 7–8 per genotype. (E) Calculation of total area under the curve (AUC) from PTTs shown in (C,D). (F) Glucose tolerance tests (GTTs) administered to mice on chow diet or HFD for 15 weeks; for chow n = 8–11 per genotype and for HFD n = 7–8 per genotype. (G) Insulin tolerance tests (ITTs) administered to mice on HFD for 16 weeks; n = 4–5 per genotype. (H) Circulating insulin in the serum of overnight fasted and 2 hr refed mice on HFD; n = 6–7 per genotype. All data are means ± SEM. PTT (C,D), GTT (F) and ITT (G) were analyzed by repeated measures two-way ANOVA. For PTT AUC in E, *p < 0.05 by two-way ANOVA. For H, serum insulin was analyzed by two-way ANOVA. ns, not significant.
