Figure 5

Proportions of MeHg-GSH, MeHg-Cys, Hg²⁺, MeHg⁺ and other species of mercury in a HepG2 cell. The total amounts of mercury were obtained by ETV-ICP-MS determination; the mount of Hg²⁺ and MeHg⁺ were obtained by chip-based on-line MSPME-microHPLC-ICP-MS analysis; the amount of MeHg-GSH and MeHg-Cys were obtained by C18 RP-HPLC-ICP-MS measurement; the normalization method was use for the calculation. (The values of 4.5%, 3.4%, 2.2%, 6.1%, 3.7% and 8.4% of MeHg-GSH were calculated based on the semi-quantified results because the concentrations of MeHg-GSH under these incubation conditions are lower than the LOQ but higher than the LOD by RP-HPLC-ICP-MS analysis).