Figure 3 | Scientific Reports

Figure 3

From: Experimental mitochondria-targeted DNA methylation identifies GpC methylation, not CpG methylation, as potential regulator of mitochondrial gene expression

Figure 3

Bisulfite sequencing of mtDNA in cells with or without mitochondria-targeted M.SssI or M.CviPI. Bisulfite sequencing of a region in the D-loop (H-strand) (a,c) and mtCOX2 gene (L-strand) (b,d) for C33A and HCT116 cells expressing a mitochondria-targeted CpG methyltransferase M.SssI (MLS-M.SssI), the catalytically inactive double mutant of M.SssI (MLS-M.SssI ∆∆, only for HCT116 cells) or empty vector control (MLS-NoED) (a,b), or a mitochondria-targeted GpC methyltransferase M.CviPI or wild-type cells (wt) (c,d). Each circle represents a CpG (a,b) or GpC (c,d) position. The percentage of methylation on each position is represented in black.

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