Figure 9 | Scientific Reports

Figure 9

From: Experimental mitochondria-targeted DNA methylation identifies GpC methylation, not CpG methylation, as potential regulator of mitochondrial gene expression

Figure 9

Mitochondrial and cellular functions in general in cells expressing mitochondria-targeted M.SssI or M.CviPI. In C33A (a,b,c,e,g) and HCT116 (a,b,d,f,h) cells stably expressing mitochondria-targeted M.SssI (MLS-M.SssI) or mitochondria-targeted M.CviPI (MLS-M.CviPI) the effect was determined of mtDNA methylation on: (a,b) mitochondrial metabolic activity (day 1) and cell proliferation (day 4), as measured by MTS; (c–f) mitochondrial superoxide (O2 •−) production, as measured with the MitoSox Red ROS probe; (g,h) sensitivity toward ROS-induced cell death, as measured with PI. H2O2 was used as a general ROS inducer, whereas menadione was used to specifically induce mitochondrial O2 •−. Each data point corresponds to the mean ± SEM of at least three independent experiments.

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