Table 1 Primer sequences.

From: Experimental mitochondria-targeted DNA methylation identifies GpC methylation, not CpG methylation, as potential regulator of mitochondrial gene expression

Target

Forward sequence (5′–3′)

Reverse sequence (5′–3′)

Cloning

AscI-DNMT1-PacI

ataGGCGCGCCATGCCGGCGCGTACCG

cagTTAATTAAGTCCTTAGCAGCTTCCTCCTCCTT

BclI-mtDNMT1-NotI

gtaTGATCACCATGGCCGGCTCCGT

ctaGCGGCCGCCTAGTCCTTAGCAGCTTCCTC

AscI-M.CviPI-PacI

aatGGCGCGCCACCTTGAAAGCGCTCG

ggcTTAATTAATATTCTAACAAATTTCCTAAATATTCTTTG

AscI-hM.CviPII-PacI

taaGGCGCGCCATGAGAACCAAGTATCGGATC

tggTTAATTAAGTAGTGCATCAGGTCCC

NotI-conII promoter-NotI

GGCCGCAGATCCATTATACGAGCCGATGATTAATTGTCAACAGC

GGCCGCTGTTGACAATTAATCATCGGCTCGTATAATGGATCTGC

q(RT-)PCR

mtND1 (RtprimerDB)

ATACCCCCGATTCCGCTACGAC

GTTTGAGGGGGAATGCTGGAGA

mtND651

GGGTGGTGGTTGTGGTAAAC

CCCCGAGCAATCTCAATTAC

mtCOX1 (RtprimerDB)

CGATGCATACACCACATGAA

AGCGAAGGCTTCTCAAATCA

mtCYTB (RtprimerDB)

AATTCTCCGATCCGTCCCTA

GGAGGATGGGGATTATTGCT

12S rRNA52

CTGCTCGCCAGAACACTACG

TGAGCAAGAGGTGGTGAGGT

16S rRNA52

GTATGAATGGCTCCACGAGG

GGTCTTCTCGTCTTGCTGTG

PGC1α

TGAGAGGGCCAAGCAAAG

ATAAATCACACGGCGCTCTT

NRF123

GGGAGCTACAGTCACTATGG

TCCAGTAAGTGCTCCGAC

TFAM23

CCGAGGTGGTTTTCATCTGT

TCCGCCCTATAAGCATCTTG

mtDNMT1 #112

TCCCTGGGCATGGCCGGCT

CTCTTTCCAAATCTTGAGCCGC

mtDNMT1 #2

CCTCCCCATCGGTTTCCG

CCAAATCTTTGAGCCGCCTG

mtDNMT1 #3

ATGGCCGGCTCCGTTCCA

“ ”

β-actin

CCAACCGCGAGAAGATGA

CCAGAGGCGTACAGGGATAG

mtDNA ratio D-loop

TCACCCTATTAACCACTCACGG

ATACTGCGACATAGGGTGCTC

nDNA ratio β-actin

TGAGTGGCCCGCTACCTCTT

CGGCAGAAGAGAGAACCAGTGA

7S DNA primer A + B115

GTGGCTTTGGAGTTGCAGTT

CAGCCACCATGAATATTGTAC

A + B215

“ ”

GAAGCAGATTTGGGTACCAC

MeDIP

D-loop_qMeDIP18

ACATAGGGTGCTCCGGCTCCA

TCCGACATCTGGTTCCTACTTCAGG

mtCYTB_qMeDIP18

TCACCAGACGCCTCAACCGC

GCCTCGCCCGATGTGTAGGA

mtCOX2_qMeDIP18

CCGTCTGAACTATCCTGCCC

GAGGGATCGTTGACCTCGTC

GAPDH_qMeDIP60

CTCTCTCCCATCCCTTCTCC

CAAGTTGCCTGTCCTTCCTA

Bisulfite sequencing

BS6_D-loop (H)13

CACATCTCTACCAAACCCC

TGGGGTGATGTGAGTTTGTT

BS6_D-loop (L)13

AGAGAGTATATTTTTGTTAAATTTT

AGGAAGAGAGACCCATCTAAACATTTTCAA

mtCOX2 (L)7

ATTGGTTATTAATGGTATTGAATTTA

CTCCACAAATTTCAAAACATTAAC

  1. Restriction sites are underlined.
  2. The 6- to 10-bp tags added to primers are indicated in bold.
Back to article page