Table 1 Peak identifications from a protein A load sample.

From: New platform for simple and rapid protein-based affinity reactions

Sample

Peak No.

Retention Time (min)

Observed mass (Da)

Standard IgG

1

15.35

149207

2

14.81

23382

101380

125840

Supernatant (original)

1

15.46

149204

2

14.93

23384

3

14.00

46894

4

12.18

23567

5

7.15

11666

6

5.99

—

Fraction 2 (IgG fraction from ProA-SpM)

1

15.61

149208

Fraction 1 (flow through fraction from ProA-SpM)

3

13.99

46893

4

12.21

23566

5

7.21

11677

6

6.14

—

  1. Fractions 1 and 2 were collected from the separation (Fig. 1(j)) from the front and back peaks, respectively. The peaks are corresponding to Fig. 1(k). The observed mass was estimated by a deconvolution of multi ions with MaxEnt1.
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