Figure 6
A high concentration of EGTA (2 mM, 5 mM and 10 mM) prevented cells from bilirubin induced enhancement of Ca2+ currents. (a) Representative traces at −10 mV for currents obtained in the presence or absence of 6 μM bilirubin (black trace) with 0.5 mM EGTA (magenta trace, left panel) and 10 mM EGTA (cyan trace, right panel) in intracellular solutions. Each current was elicited by a 40 ms test pulse to −10 mV from −100 mV. (b) The normalized VGCC current amplitude before (black bar) and during application of 6 μM bilirubin with pipette solutions containing 0.5 mM EGTA (magenta bar), 2 mM EGTA (green bar), 5 mM EGTA (orange bar) and 10 mM EGTA (cyan bar). *P < 0.05, **P < 0.01, ns, not significant, one-way ANOVA with Bonferroni post hoc test.
