Figure 7
From: MKL1 defines the H3K4Me3 landscape for NF-κB dependent inflammatory response
MKL1 recruits SET1 to activate the transcription of p65 target genes. (A) THP-1 cells were transfected with indicated siRNA followed by treatment with TNF-α (10 ng/ml) and harvested at indicated time points. ChIP assays were performed with anti-SET1. (B) THP-1 cells were transfected with indicated siRNA followed by treatment with LPS (100 ng/ml) for 6 hours. ChIP assays were performed with anti-SET1. (C) THP-1 cells were treated with or without TNF-α (10 ng/ml) for 3 hours. Re-ChIP assays were performed with indicated antibodies. (D) THP-1 cells were treated with or without LPS (100 ng/ml) for 6 hours. Re-ChIP assays were performed with indicated antibodies. (E) THP-1 cells were transfected with indicated siRNA followed by treatment with TNF-α (10 ng/ml) and harvested at indicated time points. mRNA levels of pro-inflammatory mediators were measured by qPCR. (F) THP-1 cells were transfected with indicated siRNA followed by treatment with TNF-α (10 ng/ml) for 6 hours. Protein levels of pro-inflammatory mediators were measured by qPCR. (G,H) THP-1 cells were transfected with indicated siRNA followed by treatment with LPS (100 ng/ml) for 6 hours. mRNA (G) and protein (H) levels of pro-inflammatory mediators were measured by qPCR and ELISA.
