Figure 2
From: Dentin sialoprotein facilitates dental mesenchymal cell differentiation and dentin formation
Expression of dentin sialoprotein and occludin in developing mouse teeth. At embryonic day (E) 13.5, DSP and Ocln expressions were not detected in tooth tissues (b,c) using double fluorescent immunohistochemistry, but at postnatal day (PN) 1, DSP expression (red) was observed in odontoblasts, ameloblasts and dental pulp cells (g), Ocln expression (green) was detected in these areas overlapped with DSP (h). At PN5 and PN15, expression of DSP and Ocln was apparently seen in odontoblasts and ameloblasts (l,m,q,r). However, Ocln expression was relatively wider than that of DSP. (a,f,k and p) show bright images. The cells were stained with Hoechst for nuclei (d,i,n,s). Images were merged (e,j,o,t). (a–t) show higher magnifications from the yellow boxes.
