Figure 8
From: Dentin sialoprotein facilitates dental mesenchymal cell differentiation and dentin formation
Effect of DSP domain on dental cell differentiation in vivo. (A) Lane 1, DSPf5 protein only; lane 2, DSPf5 was mixed with Affi-Gel Blue Gel Beads at room temperature for overnight. The mixture was centrifuged and supernatant was loaded onto a 7% SDS-PAGE gel; lane 3, the pellet was added to 1 x SDS-loading buffer and heated. The released DSPf5 from the compound was loaded onto a 7% SDS-PAGE gel. (B) The complex of DSPf5 coated to agarose beads was implanted into mouse dental pulp chambers. Compared to the control group after 3 week operation (Ba–c), in the DSPf5-treated group, DSPf5 was able to induce the dental pulp cell proliferation and differentiation. Dental pulp cells in the DSPf5-treated group secrete ECM (arrow) at the top “artificial hole” between resin and dental pulp. Blood vessels (BV) proliferate and migrate near agarose beads (AB). Arrowheads show that dental pulp cells differentiate and secrete ECM. b, c and e–g are enlarged from the boxes in the b’, c’ and e’–g’. In DSPf5-treated group after 5 week operation (C), ECM secreted by dental pulp cells forms a layer covering “the artificial hole” between resin and dental pup cells (Ce, f). Dental pulp cells surround the agarose beads (AB) and AB was resorbed and dental pulp cells and blood vessels invade into AB (Cg, h). A number of inflammatory cells were decreased compared to the control group. b–d and f–h are enlarged from the boxes in the b’–d’ and f’–h”. B, alveolar bone; BV, blood vessels; DP, dental pulp cells; ECM, extracellular matrix; IC, inflammatory cells; R, resin; T, teeth.
