Figure 5
From: Efficient Generation of diRNAs Requires Components in the Posttranscriptional Gene Silencing Pathway
Genetic requirements for efficient diRNA generation. (A) GU-US transgene expression in PTGS mutants. Relative expression was determined by qRT-PCR in PTGS mutants. Error bars indicate standard error of 5 independent T1 transgenic lines. (B) Relative HR rate determined by qPCR. Error bars indicate standard error of 5 independent T1 transgenic lines. (C) Detection of small RNAs. The U part of GUS gene was used as a probe. miR167 was probed as a loading control. Northern blotting image was cropped nearby signals.
