Figure 5 | Scientific Reports

Figure 5

From: Kaposi’s sarcoma-associated herpesvirus ORF34 is essential for late gene expression and virus production

Figure 5

ORF34 colocalizes with ORF24, ORF31, ORF18, ORF23, or ORF66. HeLa cells were also transfected with 2xS-tagged ORF34 (a), 6xMyc-ORF24 (b), 3xFLAG-ORF31 (c), 3xFLAG-ORF18 (d), 3xFLAG-ORF66 (e), or 6xMyc-ORF23 plasmids (f). 2xS-tagged ORF34 plasmid was transfected alone or cotransfected with 6xMyc-ORF24 (g), 3xFLAG-ORF31 (h), 3xFLAG-ORF18 (i), 3xFLAG-ORF66 (j), or 6xMyc-ORF23 plasmid (k) into HeLa cells, which were then subjected to IFA. Immunofluorescent images were obtained with an inverted confocal microscope. DNA were visualized with Hoechest 33342 staining, and 2xS-tagged ORF34 and the other ORFs (3xFLAG- and 6xMyc-) represented with red and green color, respectively. “Merge” indicates overlaid images of ORF34 (red) and another ORF (green).

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