Figure 6

Correlation between np-Au reflection at 555 nm and the observed fluorescence intensity: Fluorescent Nile Red microspheres with a diameter of 10 µm were dispersed on the surface of a np-Au feature size library (inset). The intensity from each morphology previously measured by reflection dark field spectroscopy was observed at a constant exposure time of 50 ms. The mean ensemble-average of the observed fluorescence intensity is plotted as a function of the measured reflection at 555 nm (error bars represent standard deviation). The previously measured feature size for each point is indicated at each measurement point. While dark field reflection had a linear dependence on the feature size, fluorescence intensity exhibited a non-monotonous trend with respect to feature size with an inflexion around 50 nm to 65 nm. (Red dashed line is only for visual aid and not a fit).