Figure 7
Non-classical monocytes localize to a perivascular niche. (a) Sorted CX3CR1lo and CX3CR1hi mouse monocytes associate with murine endothelial cell networks in vitro (solid white arrows) (b) The proportion of experimental CX3CR1hi monocytes within 20 µm of the vessel network compared to CX3CR1lo monocytes and a model-simulated random distribution of cells. Data presented as mean ± S.E.M. Statistical analyses were conducted using two-way ANOVA. *p < 0.05, n = 3 independent experiments. (c) Intravital imaging of CX3CR1lo and CX3CR1hi cells around PLGA implants in DWC mice 1 day post-surgery. (d,e) Distance of CX3CR1lo and CX3CR1hi cells to closest to blood vessel. Vessels were labeled with i.v. injection of rhodamine-dextran. Statistical analysis was performed using two-tailed Mann-Whitney test. *p < 0.05, n > 100 cells, across 3–4 animals per group. Scale bars, 100 µm.
