Figure 1
From: Locating the Human Cardiac Conduction System Using a 3D Model of Its Nutritious Arteries
The results of the histological and immunofluorescence examination of the SAN. (A) HE staining (scale bar = 1200 μm). (B) Masson staining (scale bar = 1200 μm). (C) Immunofluorescence staining with the anti-protein gene product 9.5 antibody (PGP9.5, the green region is the positive area). (D) Immunofluorescence staining with the anti-tyrosine hydroxylase antibody (TH, the green region is the positive area). (E) Immunofluorescence staining with the anti-connexin 40 antibody (Cx40, the green region is the positive area). (F) Immunofluorescence staining with the anti-hyperpolarization-activated cyclic nucleotide-gated channel 4 antibody (HCN4, the green region is the positive area). In C–F, cell nuclei are stained with DAPI, scale bar = 400 μm.
