Figure 3
From: A high-fat diet impairs reproduction by decreasing the IL1β level in mice treated at immature stage
Inflammasome expression in immature and mature mice after fed a HFD. Gene expression of the inflammasome components NLRP3, ASC and caspase-1 were measured in testes using qRT-PCR in G4H and G4C mice (A) and in G8C4H and G12C mice (B). Total testis protein was extracted from each group: G4H and G4C (C), G8C4H and G12C (D). Then, western blotting was used to measure the protein expression of NLRP3, caspase-1, cleaved-caspase-1 and β-actin. Western blot analysis of inflammasome-related proteins in the different groups is shown under the corresponding WB results. Densitometric ratio analyses (NLRP3, ASC, caspase-1/β-actin) were performed using Image J software. *P < 0.05 and **P < 0.01.
