Figure 4
From: A high-fat diet impairs reproduction by decreasing the IL1β level in mice treated at immature stage
IL1β inhibits the testosterone synthesis of Leydig cells. (A) The TM3 cell number stimulated by different concentrations of IL1β for 24 h, 48 h and 72 h. (B) Testosterone produced by TM3 cells under the stimulation of different concentrations of IL1β. (C) Equal decapsulated testes from 6-week-old mice and 10-week-old mice were incubated with several concentrations of IL1β for 24 h in vitro. The testosterone of the supernatants was tested using ELISA. (D) The mRNA expression of STAR, P450SCC, P45017α, 3-βHSD and 17β-HSD in TM3 cells treated with different concentrations of IL1β for 24 h. The photograph shows a representative result of three independent experiments. (E) The mRNA expression of STAR, P450SCC, P45017α, 3-βHSD and 17β-HSD in G4H, G4C, G12H and G12C testis. *P < 0.05 and **P < 0.01.
