Figure 5

Histological analysis of bone regeneration and scaffold degradation at different times after implanting operation. (A) HE staining showing a small amount of newly formed trabeculae at 2 weeks in both CM-ALs (0%) and CM-ALs (10%) groups. At week 4 and 8, coarse bone trabeculae were presented and clustered with the vascular structure clearly seen. The fibrous tissue filled defect was still observable in the CM-ALs (0%) group, while it was less visible in the CM-ALs (10%) group; (B) The quantitative analysis showed the increased new bone area with the incorporated CM-ALs and increased days. White arrows indicate areas of new bone formation, while black arrows indicate the scaffolds (Magnification: ×40). *p < 0.05 vs the CM-ALs (0%) control group.