Figure 6
From: Non cell-autonomous role of DCC in the guidance of the corticospinal tract at the midline
DCC is not detected in CST axons when they cross the midline. (A) Schematic representation of a P0 mouse sagittal section indicating the trajectory of the CST and the level of the coronal sections presented in this figure. Coronal sections of P0 Emx1::cre;Tau GFP mice (n = 3, B, C, D) and of P0 Emx1::cre;Dcc lox/lox;Tau GFP mice (n = 3, E) stained with anti-DCC (B–E), anti-GFP (B’–E’), and both labels (B”–E”). GFP staining was used as a marker of corticospinal tract axons. DCC was not detected at the CST in the pons (B,E), brainstem (D) or pyramidal decussation (F). DCC was expressed in the fasciculus retroflexus (FR, B,E), used as a positive control. Dashed lines indicate the midline. The scale bar represents 184 μm.
