Table 1 MiSeq input and post quality control (QC) data for metagenomic analysis via MG-Rast standard pipeline for the 1912 B. emiliae specimen (MG-Rast accession number: 4681440.3) and the 1942 Japanese Bemisia species (MG-Rast accession number: 4690946.3).

From: Novel molecular approach to define pest species status and tritrophic interactions from historical Bemisia specimens

 

1912 B. emiliae

%

1942 Bemisia sp.

%

Up-load count (bp)

950,823,796

 

870,198,516

 

up-load seq count

9,041,126

 

5,813,174

 

Upload: Mean Sequence Length (bp)

105 ± 54

 

149 ± 56

 

Post QC: Count (bp)

848,681,218

87.6

839,130,417

95.4

Post QC: Sequences Count

7,921,154

 

5,544,879

 

Post QC: Mean Sequence Length (bp)

107 ± 54

 

151 ± 55

 

Taxonomic Hits Distribution

Bacteria

860,366

90.4*

75,320

26

Proteobacteria

803,377

84.3*

58,567

20.2

Enterobacteriaceae

75,341

7.9

13,319

4.6

Cand. Hamiltonella

1,613

0.2

491

0.2

Cand. Portiera aleyrodidarum

545

0.06

1,228

0.4

Arsenophonus

539

0.06

n/a

n/a

Bacteroidaceae

1,642

0.2

703

0.2

Cand. Cardinium

87

<0.01

n/a

n/a

Alphaproteobacteria

142,248

14.9*

11,640

4.0

Rickettsia

1,527

0.2

915

0.3

Wolbachia

1,427

0.2

3,743

1.3*

Comamonadaceae

Acidovorax

291,123

30.1*

6,903

2.4

Eukaryota

86,636

9.1

211,856

73.1*

 Arthropoda

57,242

6.1

176,903

61.0*

  Bemisia

2,088

0.2

429

0.1

  Nasonia

5,527

0.6

64,603

22.1*

  Harpegnathos

3,064

0.3

15,369

5.2*

  Camponotus

2,858

0.3

17,345

5.9*

  Apis

2,931

0.3

9,546

3.3*

Viruses

2,036

0.2

1,522

0.5

Others

1,901

0.2

1,006

0.3

  1. A summary of taxonomic hits for Bacteria, Eukaryota, Viruses are provided. Hit abundances that differed greatly between B. emiliae and the 1942 Bemisia sp. are indicated in by ‘*’.