Table 3 Percentage (%) sequence identity of Bemisia species Wolbachia endosymbionts wsp partial gene (230 bp).

	Jap_1942	KJ648499	AJ291370	JN315980	FJ545748	KJ648498	HQ404797	GU968901	KJ648500	AJ291379	KJ648502	KJ648503
KJ648499	99.13
AJ291370^†	98.7 (100)	98.7
JN315980^†	98.7 (100)	98.7	100
FJ545748	98.7	98.7	100	100
KJ648498	98.7	98.7	100	100	100
HQ404797^†	98.26 (100)	98.26	99.57	99.57	99.57	99.57
GU968901^†	98.26 (98.11)	98.26	99.57	99.57	99.57	99.57	99.13
KJ648500	95.65	96.52	96.96	96.96	96.96	96.96	96.52	96.52
AJ291379^†	94.78 (87.74)	95.65	96.09	96.09	96.09	96.09	95.65	95.65	97.39
KJ648502	93.91	94.78	95.22	95.22	95.22	95.22	94.78	94.78	96.52	98.26
KJ648503	93.91	94.78	95.22	95.22	95.22	95.22	94.78	94.78	96.52	98.26	97.39
KJ648501	81.12	81.12	81.55	81.55	81.55	81.55	81.97	81.12	83.69	83.69	83.26	83.69

The Wolbachia wsp partial gene from the historical 1942 Bemisia specimen (‘Jap_1942’) was most similar to KJ648499, which belonged to the W2 Wolbachia strain isolated from invasive (i.e., MED, MEAM1) and native (Asia I, Asia II3, China 2) B. tabaci cryptic species complex. ‘^†’Indicates the five Wolbachia strains that had sufficient wsp gene sequence at the 5′ region to enable sequence identity comparison with the historical Japanese Wolbachia wsp gene (sequence identity acorss these 5′ end of 106 bp are indicated within parentheses).
Note: GenBank accession numbers provided for Wolbacia strains W1 (KJ648498), W2 (KJ648499), W3 (KJ648500), W4 (KJ648501), W5 (KJ648502), and W6 (KJ648503) are as reported by ref. 33.

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