Table 4 Comparison of single nucleotide polymorphism (SNP) profile of the Wolbachia coxA gene between known Bemisia tabaci cryptic species, B. afer, and the Japanese 1942 (JAP1942) Bemisia specimen.

From: Novel molecular approach to define pest species status and tritrophic interactions from historical Bemisia specimens

Nucleotide position

 

15

21

24

30

33

52

60

120

123

130

135

141

165

189

246

249

252

255

258

259

Consensus sequence

T

G

C

G

G

G

A

A

A

C

T

T

C

C

C

T

A

C

A

G

coxA 14

.

A

A

.

A

A

C

C

T

T

T

C

T

T

T

C

C

T

G

A

B. afer (ST382)

C

A

.

A

.

.

.

.

T

.

.

.

.

.

.

C

.

.

G

A

JAP1942 coxA

?

?

?

?

?

?

?

?

?

?

?

?

?

.

.

C

.

.

G

A

 

261

264

276

288

294

299

300

303

307

315

324

331

333

339

342

357

375

384

394

402

consensus sequence

C

G

C

T

T

A

G

A

A

C

T

G

A

T

T

C

A

A

G

C

coxA 14

T

.

T

A

.

T

.

.

G

.

A

A

G

C

C

.

G

G

A

T

B. afer (ST382)

T

A

.

A

C

.

A

G

G

T

.

.

.

C

.

T

G

.

.

T

1942 coxA

T

A

.

A

C

.

A

G

G

T

.

.

.

C

.

T

?

?

?

?

  1. coxA-14 sequences include B. tabaci SSA1-SG1, and B. tabaci SSA1-SG2 and are as reported in ref. 34. Consensus sequence identies are noted below. coxA missing regions for the 1942 Japanese Bemisia speciemen are between nucleotide positions 1 and 174, and from 364 to 402. Unknown SNP porfiles are indicated by ‘?’.
  2. Note: consensus sequence SNP profiles were from identical coxA_88 seqeunce. coxA sequences aligned included: HQ404793, JQ013511, B. tabaci Q(ST116), B. tabaci Asia-I (ST378, ST385, ST395), B. tabaci Asia II-1 (ST389, ST390, ST391, ST392), B. tabaci Asia II-3 (ST396), B. tabaci Asia Ii-6 (ST393, ST394), B. tabaci Asia II-7 (ST378), B. tabaci Asia II-9 (ST384), B. tabaci China-1 (ST377, ST379, ST383), B. tabaci SSA1-SG5 (coxA 88), B. tabaci SSA1-SG3 (ST424, 425), B. tabaci Australia (ST380), and B. afer NG (ST427).
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