Table 1 Microscopy and image processing details for CPMV-M and CPMV-T.

From: The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy

 

CPMV-M

CPMV-T

Data Collection

 Particles in final reconstruction

10,850

4,696

 Pixel size (Å/pixel)

1.10

1.10

 Defocus range (μm)

0.35–10.0

0.35–8.0

 Voltage (kV)

300

300

 Dose (e−/Å2)

42

42

Particle Numbers

 Autopicking

24,976

5,594

 Particle sorting

21,931

5,457

 2D classification

15,228

5,165

 3D classification

10,850

4,696

EM Refinement

 Final resolution (Å)

3.94

4.25

 Experimental B-factor (Å2)

−144.1

−185.8

 EMDB accession number

EMD-3565

EMD-3562

Model Validation

 MolProbity score

1.41 (97th percentile)

1.32 (98th percentile)

 Clashscore (all atoms)

1.77 (99th percentile)

1.51 (99th percentile)

 Ramachandran favoured

92.34%

93.45%

PDB ID

5MSH

5MS1