Figure 3
From: Novel insights into the interaction of UBA5 with UFM1 via a UFM1-interacting sequence

Structural insight into the UIS-UFM1 interaction. (A) Contacts between UFM1 (orange) and the UIS of UBA5 (blue). (B) Electrostatic surface representation of UFM1 bound to the UIS. (C) Pull down assay showing the effect of UIS mutations on binding to UFM1. (D) Charging assay of UBA5 WT or mutants (20 μM) with UFM1 WT or mutants (100 μM).