Table 3 Constants and statistics from fits of UBA5 steady-state saturation kinetics to the Michaelis-Menten model.
From: Novel insights into the interaction of UBA5 with UFM1 via a UFM1-interacting sequence
Michaelis-Menten Analysis | Data Sets | |
|---|---|---|
UBA5 WT | UBA5 H336D | |
Best-fit values | ||
Vmax (intensity/min) | 2151 | 943.1 |
Km (μM) | 28.99 | 80.35 |
Std. Error | ||
Vmax (intensity/min) | 151.9 | 38.08 |
Km (μM) | 6.670 | 9.656 |
95% Confidence Intervals | ||
Vmax (intensity/min) | 1838 to 2464 | 865.6 to 1021 |
Km (μM) | 15.26 to 42.73 | 60.71 to 100.0 |
Goodness of Fit | ||
Degrees of Freedom | 25 | 33 |
R square | 0.8503 | 0.9636 |
Absolute Sum of Squares | 1.574e + 006 | 92611 |
Sy.x | 250.9 | 52.98 |
Constraints | ||
Km | Km > 0.0 | Km > 0.0 |
Number of points | ||
Analyzed | 27 | 35 |
