Table 4 KD values and statistics from fit to one site specific binding of UFM1-UBA5.

From: Novel insights into the interaction of UBA5 with UFM1 via a UFM1-interacting sequence

One site — Specific binding

Data Sets

UBA5 WT

UBA5 H336D

Best-fit values

Bmax

47820

36944

Kd (μM)

8.853

21.90

Std. Error

Bmax

1306

1511

Kd (μM)

0.9815

3.497

95% Confidence Intervals

Bmax

45165 to 50474

33883 to 40006

Kd (μM)

6.859 to 10.85

14.81 to 28.98

Goodness of Fit

Degrees of Freedom

34

37

R square

0.9712

0.9394

Absolute Sum of Squares

3.443e + 008

4.387e + 008

Sy.x

3182

3444

Number of points

Analyzed

36

39