Figure 6
MSTN influenced cell cycles and apoptosis of adipocytes. Subcutaneous pre-adipocytes were treated with 50 ng/ml MSTN for 2 days and then harvested for further detection. (a) The effects of MSTN on the cell cycles of pre-adipocytes. Cells were stained with PI, then the cell cycles were detected by flow cytometry (left) and subsequently displayed as column charts after quantification (right), n = 3 per group. (b) The effects of MSTN on the apoptosis of pre-adipocytes. Cells were stained with PI and Annexin V, then the apoptosis rate was detected by flow cytometry (left) and displayed as column charts after quantification (right). n = 3 per group, *P < 0.05; **P < 0.01.
