Figure 5 | Scientific Reports

Figure 5

From: Identification of genes related to salt stress tolerance using intron-length polymorphic markers, association mapping and virus-induced gene silencing in cotton

Figure 5

Plant phenotypes after target genes silencing with salt and mock treatments. (A) After two weeks infiltrated with TRV2: target silenced genes, the treated plants exhibited normal growth. TRV2:GhCLA1 plants exhibited a photobleaching phenotype as indicator. TRV: 00 as the mock treatment and no-infiltration plants as CK. (B) Gene expression of five candidate genes in silenced and control plant leaves by real-time PCR analysis. The error bars were calculated based on three biological replicates using standard deviation. The cotton beta-tubulin gene (GhTub1) was used as the reference gene. “*” at P < 0.05, “**” at P < 0.01. (C) Phenotypes of target gene-silenced and control plants after the salt stress treatment. Plants were treated with 200 mM NaCl for a month (H20 as control). a-g indicate CK (no-infiltration), TRV:00 (mock treatment), TRV:C3, TRV:C4, TRV:C5, TRV:C7 and TRV:C9, respectively. Upper: photographed from plants side; Below: photographed from plants top.

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