Table 1 Summary of parameters acquired for single cells cultured in the absence and presence of Cell Tracer.

From: Dynamics of single human embryonic stem cells and their pairs: a quantitative analysis

Parameter and notation

 

No Cell Tracer

With Cell Tracer

Number of cells

N

26

22

Migration speed (μm/hr)

v

\({16.25}_{-3.9}^{+8.4}\)

\({11.51}_{-3.8}^{+3.3}\)

Step length in x (μm)

l x

\({2.5}_{-1.3}^{+2.5}\)

\({1.9}_{-0.9}^{+1.0}\)

Step length in y (μm)

l y

\({2.5}_{-1.3}^{+2.5}\)

\({1.9}_{-0.9}^{+1.0}\)

Correlation time (hr)

Ï„

\({0.6}_{-0.4}^{+0.9}\)

\({0.7}_{-0.5}^{+1.1}\)

Diffusivity (μm2/hr)

D

79.8 ± 5.2

49.1 ± 3.5

Time to first division (hr)

t div

\({4.9}_{-1.6}^{+5.1}\)

\({9.3}_{-3.4}^{+3.3}\)

Death fraction at 10 hours, %

—

0

23

Death fraction at 20 hours, %

—

4

23

  1. The entries are representative median values, with errors given by the lower and upper quartiles. The exception is the diffusivity, which is presented as a mean and 95% confidence interval. The step lengths and migration speed were calculated by averaging over all cells at all times (15 min intervals). The diffusivity was obtained using the fits to \(\overline{{L}^{2}}\) shown in Fig. 3 and the correlation time from τ = 2D/v 2 for instantaneous speeds.