Table 2 Parameters characterising the migration of hESC pairs, both in the absence and in the presence of Cell Tracer.
From: Dynamics of single human embryonic stem cells and their pairs: a quantitative analysis
Parameter and notation | Â | No Cell Tracer | With Cell Tracer | |
|---|---|---|---|---|
Type A | Type B | |||
Number of pairs | N | 10 | 12 | 17 |
Tracking time (hr) | Â | 23 | 24.25 | 31.5 |
Initial separation (μm) | s 0 | \({16}_{-1.5}^{+2.5}\) | \({17}_{-2.4}^{+5.7}\) | \({16}_{-2.4}^{+6.\,2}\) |
Final separation (μm) | s f | \({91}_{-32.3}^{+81.9}\) | \({34}_{-14.2}^{+12.6}\) | \({19}_{-4.8}^{+4.3}\) |
Maximum separation (μm) | s max | \({95}_{-14.2}^{+88.9}\) | \({98}_{-19.0}^{+52.1}\) | \({26}_{-5.0}^{+7.7}\) |
Time to \({\bar{s}}_{{\rm{\max }}}\) (hr) | T max | \({17}_{-4.4}^{+2.6}\) | \({8}_{-3.9}^{+2.8}\) | \({4}_{-2.3}^{+5.3}\) |
Speed of each cell (μm/hr) | v | \({20.0}_{-8.9}^{+13.37}\) | \({20.5}_{-8.1}^{+11.9}\) | \({15.4}_{-6.8}^{+7.2}\) |
Pair centroid speed (μm/hr) | v pc | \({15.1}_{-6.1}^{+9.0}\) | \({16.2}_{-7.1}^{+8.8}\) | \({11.7}_{-4.8}^{+6.0}\) |
Relative speed (μm/hr) | v r | \({31.1}_{-12.2}^{+17.1}\) | \({27.7}_{-11.8}^{+17.0}\) | \({16.3}_{-7.7}^{+10.9}\) |
Pair centroid correlation time (hr) | τ | \({0.5}_{-0.3}^{+0.8}\) | — | — |
Pair centroid diffusivity (μm2/hr) | D | 58.5 ± 1.8 | — | — |
