Figure 7
From: Salivary gland maturation and duct formation in the African malaria mosquito Anopheles gambiae
Secretory bodies are also observed in later adulthood. Shown are confocal images of female SGs 14 (A), or 20 (B) days post eclosion stained with the dyes WGA and DAPI and antibodies against either alpha-tubulin (A) or the saliva proteins SG6 and AAPP (B). At these late time points, large WGA-positive secretory bodies are observed (A,B, arrows), sometimes within the SC (A, inset, arrow). WGA enrichment and secretory body number varied greatly with age and among individual SGs. Both SG6 and AAPP are present in secretory bodies (Biii,vi), but newly synthesized alpha-tubulin was largely excluded (A, inset). “FB” refers to fat body tissue. Individual channels are denoted “p”, “b”, “g”, or “r”. “MIP” images are maximum intensity Z-projections. Scale bar lengths are given in microns.
