Figure 6
NAC blocked PLK2 overexpression effects on apoptosis, ROS generation, and MMP production. Mouse podocytes were treated with 30 mM HDG prior to 100 μM NAC treatment. (A,B) Overexpression of PLK2 at 24 h in the presence of HDG. (C,D) Cell apoptosis was measured by annexin V-fluorescein isothiocyanate (FITC) and PI, prior to analysis by a flow cytometry 12 h after HDG treatment. (E) ROS generation was measured by DCFH-DA fluorescent probe inflow cytometry1 h after HDG treatment. (F) MMP level was measured by JC-1 inflow cytometry1 h after HDG treatment. Overexpressing PLK2 exaggerated HDG effects. NAC attenuated HDG and PLK2 overexpressing effects. **P < 0.01.
