Figure 8 | Scientific Reports

Figure 8

From: PLK2 Plays an Essential Role in High D-Glucose-Induced Apoptosis, ROS Generation and Inflammation in Podocytes

Figure 8

PLK2 knockdown suppressed diabetes-induced apoptosis and inflammatory responses in rats. Diabetic rats (n = 3) were analyzed 24 h after shPLK2 lentiviral injection. (A–C) The urinary levels of creatinine, nitrogen and protein in diabetic rats were measured by biochemical assay. (D) Glomeruli of diabetic rats with H&E staining, PLK2 immunohistochemistry, TUNEL assay, and Nephrin staining. (E,F) Quantitative analysis and representative images of western blot. PLK2 was up-regulated in diabetic rats. Expression of apoptosis-associated markers including SIRT5, Bcl-2/Bax, p53 and cleaved caspase-3 in rats was measured. (G) Inflammatory factors TNF-α, IL-6, IL-1β, COX-2 and CXCL1 content in rats. *P < 0.05, **P < 0.01.

Back to article page