Figure 5
TbIRK co-localizes with a marker for acidocalcisomes. Co-localization of hemagglutinin (HA)-tagged TbIRK (green) with the acidocalcisomal marker TbVP1 (red) for C-terminally tagged TbIRK in procyclic form parasites (PCF, A) and N-terminally tagged TbIRK in bloodstream form parasites (BSF, B). (C) With the HA-tag at the C-terminus of TbIRK, its signal (green) partially co-localizes with TbVP1 (red, top) and partially with the ER marker BiP (red, bottom). Cells were counterstained with DAPI, shown in blue, visualizing the nuclear and kinetoplast DNA. DIC, differential interference contrast. Scale bars indicate 10 µm.
