Figure 2
From: A single biochemical activity underlies the pleiotropy of the aging-related protein CLK-1
2,4-DHB supplementation restores UQ biosynthesis in clk-1 mutants. HPLC-UV chromatograms of quinone extracts from worms with the indicated genotypes. Elution times for DMQ9 and UQ9 are indicated. At the first larval stage, the worms were transferred onto plates supplemented, or not, with DHB. They were fed the UQ-deficient bacterial strain GD1 and grown to the adult stage before being harvested for quinone extraction. Whole worm homogenates with 250 µg of protein were used, expect for the clk-1(qm30) mutants exposed to DHB at 0.5 mM in the plate where a homogenate with 440 µg of protein was used for HPLC analysis. Treatment with DHB restored UQ biosynthesis in clk-1(qm30) mutants in a dose-dependent manner, but showed no significant effect on the wild-type (N2).
