Figure 3

The behavioural defects of clk-1(qm30) mutants are fully rescued by 2,4-DHB. (a) clk-1 mutants pump at a significantly slower rate than the wild type. 1 mM 2,4-DHB fully rescues the slow pumping rates of clk-1. At this concentration, 2,4-DHB had no effect on the wild type. The bars represent the mean number of pumps per minute. (b) clk-1 mutants have a significantly lengthened defecation cycle length. 1 mM 2,4-DHB fully rescues the slow defecation phenotype of clk-1. 2,4-DHB had no effect on the wild type. The bars represent the mean defecation cycle of animals that have been scored for three consecutive defecation cycles each in the case of non-treated clk-1(qm30) mutants and for five consecutive defecation cycles for all the other conditions. The error bars represent S.E.M. (n ≥ 20 animals for each condition). The asterisks indicate that the data are significantly different from that of the wild-type. All differences were significant at p < 0.0001 by a t-test.