Figure 1 | Scientific Reports

Figure 1

From: Amide hydrogens reveal a temperature-dependent structural transition that enhances site-II Ca2+-binding affinity in a C-domain mutant of cardiac troponin C

Figure 1

Mutation D145E affects the chemical environment. (a) Superposition of 1H-15N-HSQC NMR spectra for Ca2+-bound HcTnC WT (red) and D145E (blue) reveals the most perturbed residues in the D145E protein at 25 °C. (b) Chemical-shift perturbation (CSP) plots for the N- and C-domains as a function of residue. Dashed lines show the average and one and two s.d. above the average. Residues with CSP values ≥ 2 s.d. are shown by yellow backbones on HcTnC in c, the WT NMR structure (PDB: 1AJ4) for the C-domain. Ca2+ ions are shown as green spheres and D145 as a green stick.

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