Figure 4 | Scientific Reports

Figure 4

From: Amide hydrogens reveal a temperature-dependent structural transition that enhances site-II Ca2+-binding affinity in a C-domain mutant of cardiac troponin C

Figure 4

Residues with non-linear temperature coefficients for amide hydrogens are sampling a different chemical environment above the break. (a,b) Ca2+-binding sites III and IV reveal multiple residues with non-linear temperature dependence. Residues listed to the left of each panel are located before or after the cartoons in panels a and b (NL – nonlinear, L – linear and NA – not analysed). (c,d) Collection of 1H chemical shifts with nonlinear behavior for residues located within sites III and IV, respectively. Numbers beside line segments in (c–d) represent temperature coefficients in ppb/°C. Calcium ions (green) are depicted in both sites but available evidence indicates they are bound only weakly (or not at all) in sites III and IV of D145E.

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