Figure 8

Signaling to NF-κB by different PKD2 mutants in oxidative stress conditions. (a) HeLa cells were co-transfected with control vector, indicated PKD2 expression constructs and NF-κB-luciferase and β-Galactosidase reporters. 4 hours after transfection, cells were stimulated with 500 µM H₂O₂. 18 hours after stimulation cells were lysed and reporter gene assays performed. Shown is the relative increase between untreated and treated sample. The asterisk indicates statistical significance (unpaired t test); Y717F: p = 0.0002; S706A/S710A: p = 0.0001. (b) HeLa cells were co-transfected with control vector, PKD2 wildtype or LNQ.RNK expression constructs and NF-κB-luciferase and β-Galactosidase reporters. 4 hours after transfection, cells were stimulated with 500 µM H₂O₂. 18 hours after stimulation, cells were lysed and reporter gene assays performed. Shown is the relative increase between untreated and treated sample.