Table 1 Kinetic parameters for the different PKD preparations, values shown are mean ± SD.

From: Differential regulation of PKD isoforms in oxidative stress conditions through phosphorylation of a conserved Tyr in the P+1 loop

parameter

Km (µM)

Vmax (pmol phosphate/min)

kcat (pmol phosphate/min/pmol PKD)

Conditions:

PDB

Ox. stress

PDB

Ox. stress

PDB

Ox. stress

PKD1 WT

39.8 ± 5.4

42.5 ± 4.2

125.8 ± 6.4

64.87 ± 2.5

128.4

66.2

PKD2 WT

57.5 ± 11.7

47.8 ± 7.4

130.8 ± 6.6

98.91 ± 3.55

126.9

96

PKD2 Y717F

337 ± 62.7

371.1 ± 10.6

109.2 ± 55.5

49.19 ± 3.98

106

47.8

PKD2 LNQ.RNK

97.7 ± 12.8

101.2 ± 8.8

203.4 ± 15.6

68.29 ± 3.52

197.5

66.3

Back to article page