Figure 2
From: Frequent GU wobble pairings reduce translation efficiency in Plasmodium falciparum
GU wobble pairing reduces GFP signal intensity. (a) % GFP+ cells in NF54 culture transfected with different GFP sequence variants. The GFP+ populations are determined in trophozoites population by flow cytometry analysis (n = 4). Major difference can be seen between GFPWob and GFPNon wob. Linear regression analysis between % GFP+ population and (b) averaged codon usage frequency, (c) averaged codon frequency/tRNA gene, (d) rare shift value, (e) % GC3 - codons with G or C in the 3rd position, (f) % A3, (g) % T3, (h) % G3, (i)% C3, (j) % wobble codon, (k) % non-GU wobble codon and (l) % GU wobble codon. (m) Shows a summary table for all the linear regression analyses. GU wobble content in a sequence is the best predictor of GFP signal intensity as indicated by the R2 value. Error bar = S.D.
