Figure 3
From: Frequent GU wobble pairings reduce translation efficiency in Plasmodium falciparum
High GU wobble content reduces translation of endogenous sequence. (a) % GFP+ cells in NF54 culture transfected with native and recodonized PF3D7_0202400 C-terminus sequence fused to GFP (n = 3, mean ± s.d.). (b) The GFP+ populations are determined in trophozoites population by flow cytometry analysis. The entire parasite population generates higher GFP signal when transfected with the recodonized sequence. (c) Relative copy number of gfp transcript against fructose biphosphate aldolase transcript using cDNA from the transfectants (n = 3, mean ± s.d.). (d) Western blot analysis on the transfectants with native and recodonized sequence using antibodies against GFP and Pfhsp70 as loading control. There are more GFP fusion proteins detected in transfectants with recodonized sequence. Full-length blots are shown also as supplementary figure. (e) Shows the respective wobble codon content in the native and recodonized sequence. Native sequence has more GU wobble codon.
