Figure 5
From: Frequent GU wobble pairings reduce translation efficiency in Plasmodium falciparum
Highly expressed genes have lower GU wobble content. (a) The GU wobble content in the top 5% expressed genes at 10, 20, 30 and 40 hours post invasion. Top 5% expressed genes were determined from RNAseq data. The plot shows the mean ± s.d., dotted line represents genome average. (b) Comparison between the total wobble codon usage frequencies in top 5% expressed genes and the genome. (c) Upper: The GU wobble content in (red) all genes in the genome, (blue) in genes encoding 40S ribosomal proteins and (cyan) 60S ribosomal proteins of five Plasmodium spp., P.f.: Plasmodium falciparum, P.b.: Plasmodium berghei, P.y.: Plasmodium yoelii, P.k.: Plasmodium knowlesi, P.v.: Plasmodium vivax. All t-test analyses between genome and 40S/60S in all five species show significant difference between the means. Lower: The A+T content in the different genomes. 40S and 60S ribosomal genes use less GU wobble codon but the extent is dependent on the genomic A+T content.
