Figure 2
From: Elevated levels of Wnt signaling disrupt thymus morphogenesis and function
Defective thymus development due to TEC-intrinsic β-catenin overexpression. (A) Ectopic location of the thymic lobes in Foxn1::β-catenin-transgenic mice. Foxn1-expressing thymic lobes are labelled blue by wholemount X-Gal staining. (B) Ectopic location of thymic lobes and parathyroid in E15.5 β-catenin-transgenic embryos, visualised by staining for β-catenin (upper panels 20x panoramic view, lower panels thymus tissue at 40x magnification with DAPI counterstain). (C) RNA in situ hybridization for Axin2 on thymus sections of E15.5 embryos. (D) Activity of the BATgal reporter allele in the thymus of E15.5 embryos. (E) Development of the parathyroid and thymus of wild-type mice at E12.5 examined by H&E staining and RNA in situ hybridization with the indicated gene-specific probes. (F) Development of the parathyroid and thymus of mutant mice at E12.5 examined as in (E). A numerical key for the histological labels can be found in Fig. 3.
