Figure 3 | Scientific Reports

Figure 3

From: Biochemical analysis of leishmanial and human GDP-Mannose Pyrophosphorylases and selection of inhibitors as new leads

Figure 3

Determination of LdGDP-MP, LmGDP-MP and hGDP-MP kinetic constants. (a,c,e) Lineweaver Burk double reciprocal plots 1/V = f(1/[Man-1-P]) of LdGDP-MP (a), LmGDP-MP (c) and hGDP-MP (e). GTP concentration was held constant at 150 µM for LdGDP-MP and 80 µM for LmGDP-MP and hGDP-MP. The results expressed correspond to the mean of three independent experiments ± SD. (b,d,f) Lineweaver Burk double reciprocal plots 1/V = f(1/[GTP]) of LdGDP-MP (b), LmGDP-MP (d) and hGDP-MP (f). Man-1-P concentration was held constant at 150 µM for LdGDP-MP and 80 µM for LmGDP-MP and hGDP-MP. The results expressed correspond to the mean of three independent experiments ± SD. (g) Kinetic constants (V m , K m , k cat , k cat /K m ) of LdGDP-MP, LmGDP-MP and hGDP-MP for both substrates Man-1-P and GTP.

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