Figure 4
Determination of LdGDP-MP, LmGDP-MP and hGDP-MP mechanisms of reaction. (a and b) Lineweaver Burk plots 1/V = f(1/[Man-1-P]) (a) and 1/V = f(1/[GTP]) (b) of LdGDP-MP. Constant concentrations of GTP (a) and Man-1-P (b) were held at 10 µM, 20 µM, 40 µM and 150 µM. The results expressed correspond to the mean of three independent experiments ± SD. (c and d) Lineweaver Burk plots 1/V = f(1/[Man-1-P]) (c) and 1/V = f(1/[GTP]) (d) of LmGDP-MP. Constant concentrations of GTP (c) and Man-1-P (d) were held at 5 µM, 10 µM, 20 µM and 50 µM. The results expressed correspond to the mean of three independent experiments ± SD. (e and f) Lineweaver Burk plots 1/V = f(1/[Man-1-P]) (e) and 1/V = f(1/[GTP]) (f) of hGDP-MP. Constants concentrations of GTP (e) and Man-1-P (f) were held at 10 µM, 20 µM, 40 µM and 80 µM. The results expressed correspond to the mean of three independent experiments ± SD.
