Figure 3

Obestatin-induced insulin secretion is abolished in GHS-R null mice. Overnight-fasted WT and Ghsr ^−/− mice were i.p. injected with saline or 0.5 µmol/kg obestatin, and 2 g/kg glucose was gavaged. Blood glucose in WT (A) & Ghsr ^−/− (C) and plasma insulin in WT (B) & Ghsr ^−/− (D) was measured at 0, 15 and 60 min after injection. ^#P < 0.05 saline/obestatin treatment at 15 min or 60 min vs saline/obestatin treatment at 0 min, *P < 0.05 saline vs obestatin for each time point, n = 3–5. Overnight-fasted WT and Ghsr^−/− mice were fed Ad. Lib. chow diet after saline/obestatin injections. Blood glucose in WT (E) & Ghsr ^−/− (G) and plasma insulin in WT (F) and Ghsr ^−/− (H) was measured at 0, 30 and 60 min after injection. ^#P < 0.05 saline/obestatin treatment at 30 min or 60 min vs saline/obestatin treatment at 0 min, *P < 0.05 saline vs obestatin for same time point, n = 6–8.