Figure 4
LAMP assay targeting the SbGP/MPVcpn60 gene applied to plant DNA extracts. 1, S31b-L-MA; 2, 289-L-MA; 3, P83-L-SLP; 4, P86-L-SLP; 5, healthy strawberry DNA; 6, healthy C. roseus DNA; 7, mixture of 106 copies each of all non-target plasmid templates (see Table 3); 8, no template control. (a) Reactions were viewed under ultraviolet light using a transilluminator after amplification using the Genie instrument. (b,d) Annealing and Amplification curves respectively, for the same samples using Isothermal detection chemistry. (c) Amplification curves for the same samples using calcein-based detection method.
